PHARMACEUTICALS AND DRUG DEVELOPMENT

The drug discovery and drug development process involves in vitro and in vivo experiments to evaluate the efficiency of candidate molecules (antibiotics - antibacterials, antivirals, anticancers, etc.). Efficiency evaluation methods include ATP measurements that take up to 2-4 days to obtain readable-results. By using our ATP-quantification kits containing ApiOne®, the turn-around time can be reduced by 30-40%. For more details, read through our documents or look at the performance of ApiOne®.

HYGIENE AND BIOMEDICINE

Bioluminescence tests are frequently used for the determination of bacterial ATP present in the biological samples, where the extracellular ATP should be selectively degraded, leaving the bacterial ATP unaffected. The inability to hydrolyze extracellular ATP hinders the bioluminescence methods from analyzing biological samples like serum and urine. Since ApiOne® can completely eliminate extracellular ATP and its analogues, it makes it possible to detect low levels of bacteria. In short, using our ATP diagnostic kits allows the detection of infections at very early-stages. Apart from this, rapid ATP elimination activity of ApiOne® helps robust identification of contaminated surfaces, including sterilized catheters, surgical equipment, etc.

DNA SEQUENCING

At the end of every sequencing cycle in Pyrosequencing, all the unincorporated nucleotides and excess ATP must be eliminated by apyrase. Commercial apyrase enzymes available today have problems in achieving complete degradation of ATP and ATP by-products due to the substrate specificity and batch-to-batch variations. This affects DNA sequencing efficiency of long strands. Our patent and data shows how ApiOne hydrolyses all the ATP compared to its counterpart and has the potential to significantly improve the performance of Pyrosequencing.   

glass_water

FOOD AND BEVERAGE INDUSTRY 

ATP molecules are present in all living cells, whose concentration is fairly constant per cell. Therefore measurement of ATP content in a sample can be used as a proxy to determine the number of viable cells. Ultrasensitive ATP measurements of luminescence methods could previously not produce accurate results due to the inefficient activity of conventional apyrases. As described in our product description, usage of ApiOne® can make bioluminescence assays can increase the sensitivity and enables detection of low ATP levels. Therefore, ATP-based bioluminescence assays are very useful in food and beverage, diary, and water industries has many advantages. Please see the documents for more details.